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1.
Arq. bras. med. vet. zootec ; 66(6): 1946-1950, 12/2014.
Article in English | LILACS | ID: lil-735781

ABSTRACT

Este trabalho objetivou identificar os atributos que influenciam a tomada de decisão para a compra de carne bovina, além do conhecimento e atitude dos consumidores em adquirir carne com certificação de origem, assim como os principais benefícios e dificuldades para a comercialização desse produto. Foram entrevistados 197 consumidores e nove gerentes de supermercados no Distrito Federal, em maio de 2011. Foi realizada a descrição das variáveis e aplicados o teste do qui-quadrado e o Exacto de Fischer, utilizando o pacote estatístico SPSS 17.0. A aparência visual da carne é o atributo que mais influencia a decisão de compra dos consumidores. A maioria dos entrevistados está disposta a pagar mais pela carne com certificação de origem. Esta pesquisa sugere que a escolaridade é o fator que mais influencia a disposição para compra de carne com certificação de origem. Os gerentes de supermercados salientam a fidelização de clientes mais exigentes como o maior benefício, e a falta de divulgação da rastreabilidade bovina para a população como a principal dificuldade para a comercialização...


Subject(s)
Humans , Good Manufacturing Practice Certificate , Meat Industry , Consumer Product Safety , Consumer Behavior/statistics & numerical data , Eating
2.
Braz. j. med. biol. res ; 28(6): 693-8, Jun. 1995. graf
Article in English | LILACS | ID: lil-154940

ABSTRACT

The effective size of colicin Ia channel was tested by a recently described method 9FEMS, Microbiology and Immunology (1992). 105: 93-100) in which the nonelectrolyte molecules with different hydrodynamic diameters (0.52 to 5.0nm) were used as molecular tools. We have shown that despite low conductance (55-105 pS at 1.5 MKCl, pH 7.0) the ion channels formed by colicin Ia have a fairly large water pore diameter equal to 1.66-1 1.88nm. The results are discussed in terms of an energetic barrier for ions passing into the channel lumen


Subject(s)
Lipid Bilayers/pharmacology , Ion Channels/pharmacology , Colicins/pharmacology , Colicins/toxicity
3.
Braz. j. med. biol. res ; 25(1): 75-9, 1992. ilus
Article in English | LILACS | ID: lil-109004

ABSTRACT

Antigen F1 is a protein of 17 kDa produced by Yersinia pestis it is cultured at 37 grade C. When incorporated into planar lipid bilayer membranes this protein induces fluctuations on membrane conductance typical of the formation of ionic channels. These fluctuations reveal two distinct unitary conductance sizes, one in the range of 800 to 1400 pS and the other in the range of 140 to 600 pS. Zero current potential measaurements in the presence of a salt gradient show that the channell is not significantly ion selective. The reversal potential measured in the presence of 0.5 MKCl on the cis side and 0.1 MKCl on the trans side was 3.58 ñ 3.98 mV (N=7). The non-selectivity of the channel, in addition to its large conductance, suggests that it forms large aqueous pores. The present results, taken together with other data showing that nantigen F1 inhibits the activity of phagocytic cells, suggest that antigen F1 acts by forming aqueous pores in the membrane of these target cells


Subject(s)
Bacterial Proteins/metabolism , Lipid Bilayers/metabolism , Yersinia pestis/metabolism , Ion Channels , Temperature
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